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1. Glossary

TermExplanation
ACCActive Cell Count. The ACC cartridge measures metabolically active cells using an enzymatic assay. ACC is the total number of active cells inside the ACC gate.
CBCColiform Cell Count. Number of coliform cells counted in the sample.
CartridgeRefillable container of reagents needed for BactoSense
operation
EBCE. coli Cell Count. Number of E. coli cells counted in the sample.
FCSFlow Cytometry Standard (FCS) is a data file standard for
the reading and writing of data from flow cytometry
experiments. The FCS specification has traditionally been
developed and maintained by the International Society for
Advancement of Cytometry (ISAC). FCS used to be the
only widely adopted file format in flow cytometry.
FL1Fluorescence signal 1 (530 nm)
FL2Fluorescence signal 2 (715 nm)
FSCForward scatter, also known as Small Angle Light
Scattering. A detector measures the light scattered from
microorganisms and other particles at small angles.
GateGates are tools for data analysis of a subset of data points
in flow cytometry.
HNAHigh Nucleic Acid. Bacteria with a large amount of DNA
which produce a strong fluorescence emission.
HNACHigh Nucleic Acid Count. The number of HNA bacteria
inside the TCC or ICC gate, and above the HNA / LNA
limit.
HNAP [%]High Nucleic Acid Percentage.
The percentage of HNA bacteria relative to the cell count.
ICCIntact Cell Count. Total number of intact bacteria inside of
the ICC gate.
LNALow Nucleic Acid. Bacteria with a lower amount of DNA
which produce a weaker fluorescence emission than HNA
bacteria.
LNACLow Nucleic Acid Count. The number of LNA bacteria
inside the TCC or ICC gate below the HNA / LNA limit.
Manual Sampling DeviceConnection module, allowing manual sampling with the
BactoSense Online.
NBCEnterococcus Cell Count. Number of Enterococcus cells counted in the sample.
Online Sampling DeviceConnection module to a water line, allowing online
sampling with the BactoSense Online.
SSCSide scatter, also known as Large Angle Light Scattering. A
detector measures the light scattered from
microorganisms and other particles at 90 degrees.
TCCTotal Cell Count. Total number of bacteria detected inside
the TCC gate.